Journal: OncoTargets and therapy

Article Title: Overexpression of ETV4 protein in triple-negative breast cancer is associated with a higher risk of distant metastasis

doi: 10.2147/OTT.S66692

Figure Lengend Snippet: Relative expression comparison for 84 metastasis-related genes between triple-negative breast cancer (TNBC) and luminal A breast cancer tissue detected using a Human Tumor Metastasis RT 2 Profiler™ PCR Array. Notes: The figure depicts a log transformation plot of the relative expression level of each gene (ΔΔCt) between TNBC (y-axis) and luminal A (x-axis). The red lines indicate a three-fold change in gene expression threshold. Abbreviations: PCR, polymerase chain reaction; ETV4, ETS translocation variant 4; MMP, matrix metalloproteinase.

Article Snippet: Real-time PCR was then performed on each sample using the Human Tumor Metastasis RT 2 ProfilerTM PCR Array (SuperArray Bioscience) in an OpticonTM DNA Engine ABI Prism system (Applied Biosystems, Foster City, CA, USA), according to the manufacturer’s instructions.

Techniques: Expressing, Comparison, Transformation Assay, Gene Expression, Polymerase Chain Reaction, Translocation Assay, Variant Assay

Journal: Molecular Oncology

Article Title: KLK6‐regulated miRNA networks activate oncogenic pathways in breast cancer subtypes

doi: 10.1016/j.molonc.2016.03.008

Figure Lengend Snippet: The effect of KLK6 transfection on miRNA‐mediated signaling pathways. Panels of MAPK signaling and related factors were assayed for expression changes between KLK6 overexpressing and mock cells. In general, expression of mRNAs encoding for transcription ...

Article Snippet: MAPK mRNA profiling The cDNA was synthesized as previously and was added to the MAPK Signaling Pathway RT 2 ProfilerTM PCR array (Qiagen/SuperArray Biosciences) as per the manufacturer's protocol.

Techniques: Transfection, Protein-Protein interactions, Expressing